Regulatory

Part:BBa_K656010:Design

Designed by: Evan Clark, Julius Ho, Eli Moss, Jesse Palmer   Group: iGEM11_Brown-Stanford   (2011-09-19)

Anabaena pSac Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Anabaena separates the process of photosynthesis and nitrogen fixation spatially, into two cell types. Heterocysts are cells responsible for forming an anaerobic environment in which nitrogen fixation can occur. The more numerous cell type, vegetative cells, are photosynthetic and capable of producing excess sugars.

The promoter we have modeled our part after is present in the Photo-system I of Anabaena 7120, and therefore its activity is both constitutive and confined to vegetative cells. This was done to confine sucrose secretion to only vegetative cells, as heterocysts are not producing sucrose through photosynthesis. A similar approach has been shown to successfully limit heterologous expression to vegetative cells in another species of cyanobacteria, Nostoc punctiforme. [1]

Source

Anaebaena PCC 7120

References

[1] Argueta C, Yuksek K, and Michael Summers. Construction and use of GFP reporter vectors for analysis of cell-type-specific gene expression in Nostoc punctiforme. Journal of Microbiological Methods 59 (2004) 181-188.